Inflammatory cell response in capsulotomies obtained with femtosecond laser-assisted cataract surgery

Session Details

Session Title: Femtocataract I

Session Date/Time: Sunday 14/09/2014 | 08:00-10:00

Paper Time: 09:03

Venue: Boulevard A

First Author: : L.Mastropasqua ITALY

Co Author(s): :    R. Calienno   L. Toto   C. Curcio   P. Mattei   A. Mastropasqua   G. Falconio

Abstract Details

Purpose:

to compare inflammatory cell response of capsulotomies specimens obtained with femtosecond laser–assisted cataract surgery and manual continuous curvilinear capsulorhexis.

Setting:

Ophthalmology Clinic, University 'G. d'Annunzio'Chieti-Pescara

Methods:

Seventy capsulorhexes obtained using a conventional manual technique and femtosecond laser with different energy settings were divided into 5 groups as follows: Group 1 (14 capsulorhexes) obtained with the manual technique, Groups 2 (14 capsulorhexes) obtained with the femtosecond laser at 6.0 μJ, Group 3 (14 capsulorhexes) at 7 μJ, Group 4 (14 capsulorhexes) at 10.0 μJ and Group 5 (14 capsulorhexes) at 13.0 μJ. After treatment capsulorhexes were prepared for immunohistochemistry evaluation. The expression of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) was investigated in all samples.

Results:

Immunohistochemical staining showed very few NF-κB positive cells in samples of manual technique compared to total number of cells. The percentage of NF-κB positive cells was significantly increased in samples treated with femtosecond laser at 13 µj with respect to manual technique (p=0.0005).

Conclusions:

Inflammatory cell response of capsulorhexes was related to laser pulse energy and was comparable to manual capsulorhexis for low energy settings.

Financial Interest:

NONE