Prophylactic effect of topical silica nanoparticles as a novel anti neovascularization agent for inhibiting corneal neovascularization following chemical burn

Session Details

Session Title: Cornea Medical

Session Date/Time: Tuesday 16/09/2014 | 14:00-16:00

Paper Time: 14:54

Venue: Capital Hall A

First Author: : E.Delrish IRAN

Co Author(s): :    M. Mohammadpour   M. Jabbarvand   H. Hashemi        

Abstract Details

Purpose:

To investigate the prophylactic effect of topical silica nanoparticles as a novel anti neovascularization agent for inhibiting corneal neovascularization following chemical burn.

Setting:

Nano-Ophthalmology Department, Stem Cells Preparation Unit, Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences.

Methods:

A total number of 20 corneas of 10 Wistar Albino rats were included in this study. Silver nitrate cauterization was used to the central cornea for 5 seconds to induce corneal neovascularization. They were randomly allocated to case and control groups (10 eyes in each group). Silica nanoparticles (SiNPs) were synthesized by the reverse microemulsion method. SiNPs drop 1mg/ml was started in 10 eyes and artificial tear drop was started in control group (10 eyes) immediately after chemical cauterization. Video-based photography was performed before and after treatment. Corneal Image analysis was performed on each cornea using an image analysis software program. All rats were euthanized and the eyes were sent for histopathologic exams 14 days after chemical cauterization.

Results:

SEM images showed spherical shapes of particles. The mean size and polydispersity index of prepared SiNPs were 30.1±5.6 nm and 0.254±0.11, respectively. The mean vascularized corneal area in case group was 21% of total corneal area, and 85%in control group 14 days after chemical cauterization (P<0.05). There was significantly more extensive intrastromal vascularization in control rather than case group in histopathologic examination (P<0.05).

Conclusions:

SiNPs is an effective modality for inhibiting corneal neovascularization following chemical burn in experimental model. Further investigations are suggested for evaluation of its safety and efficacy in human experiments.

Financial Interest:

NONE