PI3K-inhibitor alkylphosphocholin erufosin mitigates posterior capsular opacification in the human capsular bag alone and when soaked into intraocular lens implants

Session Details

Session Title: PCO

Session Date/Time: Monday 15/09/2014 | 14:30-16:00

Paper Time: 15:34

Venue: Capital Hall B

First Author: : C.Wertheimer GERMANY

Co Author(s): :    U. Brandlhuber   D. Kook   A. Kampik   K. Eibl-Lindner     

Abstract Details

Purpose:

Posterior capsule opascification is dependent on growth factors. Phosphoinositide 3-kinase (PI3K) is a downstream protein kinase of different growth factors including EGF, FGF and TGFbeta. Alkylphosphocholins (APC) are potent inhibitors of PI3K and it was shown before, that APC can inhibit growth of immortalised lens epithelial cells in vitro, without showing toxicity to other ocular cells. Furthermore, intraocular lenses (IOL) can be loaded with APC using lens soaking. To assess the invitro effect of both, APC and APC-loaded IOLs the human capsular bag model was used.

Setting:

In vitro. Cell and molecular biology laboratory, Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany

Methods:

For the human capsular bag model, 24 cadaver eyes from 12 human donors underwent sham cataract surgery. The capsular bag was removed and transferred into cell culture. The tissue was exposed to the IC50 of APC alone for 72 hrs, solvent only served as control. Cellular growth was observed over 60 days and foto-documented. Furthermore IOLs were incubated with APC, washed in phosphate-buffered saline, dried overnight. Intraocular lenses of the same lot served as uncoated controls. Those IOLs were implanted into the capsular bags, transfered to cell culture and observed until confluence. For both settings the time until confluence of the capsular bag was measured.

Results:

APC increased the time until confluence of the capsular bag significantly compared to controls (p<0.01). When coated and soaked to lenses the effect was even more pronounced (p<0.01).

Conclusions:

These promising in vitro results in the human capsular bag model might highlight a future clinical use of APC and APC soaked IOLs for prophylaxis of posterior capsule opascification. Further studies are warranted to evaluate this potential.

Financial Interest:

NONE