Posters

The effect of pentadecapeptide BPC-157 on healing of perforative corneal injury in rats

Poster Details

First Author: S.MASNEC CROATIA

Co Author(s):    M. Kalauz   T. Kuzman   R. Kordić   I. Škegro   B. Cerovski   R. Iveković

Abstract Details

Purpose:

To evaluate the effect of human gastric pentadecapeptide BPC-157 on healing of perforative corneal injury in rats.

Setting:

1 University Hospital Center Zagreb, Department of Ophthalmology, Zagreb, Croatia 2 University Department of Ophthalmology, University Hospital Center „Sestre milosrdnice', Zagreb, Croatia 3 Department of Pharmacology, School of Medicine, University of Zagreb, Croatia 4 Department of Pathology, School of Medicine, University of Zagreb, Croatia

Methods:

The study included 100 Male Wistar albino rats, 280-320 g body weight, divided in 4 groups (n=25). The randomization protocol was generated by the Random Allocation Software Version 1.0, 2004 (M. Saghaei, MD, Department of Anesthesia, Isfahan University of Medical Sciences, Isfahan, Iran). Penetrant linear incision 2mm was performed under deep anesthesia with ketamine, 20 mg/kg i.p. and diazepam, 6 mg/kg , along with topical anesthetic oxibuprocaine 0.4%, 2 drops on left cornea paralimbal on five o'clock with incision knife 20 Gauge MVR (Bausch&Lomb) under 45°, under operating microscope. Left eye was operated in aseptic conditions by the same surgeon. Lesions were stained by standard 10 % flourescein and photographed. Animals were medicated as follows: distilled water (control group) or BPC 157 2pg/ml, 2ng/ml, 2µg/ml, 2 drops per rat' s left eye started immediately after injury induction, every 8 hours up to 120 hours . Lesions were photographed before application or sacrifice (at 24, 48, 72, 96, 120 h). Healing process was analysed clinically using Fluorescein test, Seidel test, image analysis software (SFORM software (VAMSTECH, Zagreb, Croatia)., slitlamp and pathohistologically using standard tissue analysis.

Results:

Throughout 120h-period a steady recovery is noted in controls. Healing was significantly accelerated in eyes on µg- or ng-topical regimen of BPC 157 (p<0.05) during all intervals.

Conclusions:

BPC 157 was shown to be effective in promoting healing of corneal layers in rats. Results were dose dependent FINANCIAL INTEREST: NONE