Posters

Analysis of aqueous humor proteome after femtosecond laser-assisted cataract surgery

Poster Details

First Author: M.Kim SOUTH KOREA

Co Author(s):    H. Kim   K. Kim   J. Kim   M. Kim   H. Tchah        

Abstract Details

Purpose:

To analyze proteins which are differentially expressed between traditional manual cataract surgery and femto-second laser assisted cataract surgery (FLACS).

Setting:

Department of Ophthalmology, Asan Medical Center, University of Ulsan, College of Medicine, Seoul, Korea

Methods:

5 eyes of 5 patients underwent conventional cataract surgery and 5 eyes of 5 patients underwent FLACS were included. Aqueous humors at each group were analyzed through proteomics approach using high resolution Liquid chromatography-Mass spectrometry (LC-MS) system. To examine the function of proteins, gene ontology and KEGG pathway analysis were performed.

Results:

Total 445 and 457 proteins were observed in conventional and FLACS group, respectively. 36 proteins including heat shock protein and proteasome were observed only in conventional group. 48 proteins including immunoglobulin, complement, and coagulation factor were observed only in FLACS group. In the gene ontology analysis, in the biological process, NF-kappa signaling, WNT signaling, and MAPK cascade were observed in conventional group. In FLACS group, platelet degranulation, blood coagulation, and complement activation were observed. In the KEGG pathway analysis, proteasome, biosynthesis, and metabolism were dominant in conventional group. In FLACS group, complement and coagulation cascades and, platelet activation were dominant.

Conclusions:

There were biological differences between two methods. In conventional group, proteins related to reconstruction and remodeling were highly enriched. In FLACS group, proteins related to inflammation and immune reactions were highly enriched. These proteome data can be applied to diagnose other ocular diseases and monitor the prognosis after surgery.

Financial Disclosure:

None