Posters

A 10 year surveillance report of in vitro antimicrobial susceptibility of bacterial keratitis isolates and normal ocular commensals from eastern India in 2007-2017

Poster Details

First Author: J.Sengupta INDIA

Co Author(s):    A. Khetan   S. Saha                 

Abstract Details

Purpose:

To report the antibiotic susceptibility profile of bacterial keratitis isolates and commensal microbial flora of conjunctival cul de sac isolated over the last 10 years (2007-12 & 2013-17).

Setting:

Tertiary care eye hospital of eastern India

Methods:

Laboratory records of all consecutive cases of bacterial corneal ulcers and normal commensals were retrospectively reviewed. Data noted included microorganism isolated and antibiotic culture sensitivity to the fourth generation fluoroquinolones (moxifloxacin, gatifloxacin), fluoroquinolones (ofloxacin and ciprofloxacin), cefazolin, and to the aminoglycosides (tobramycin and gentamicin) was performed using by the Kirby-Bauer disc diffusion method.

Results:

980 bacterial isolates were identified from corneal ulcer (618 cases), endophthalmitis (175 cases) and conjunctival swab culture (187 cases). Of these 685 (70%) were Gram-positive and 295 (30%) were Gram-negative. Staphylococcus epidermidis (327, 47.7%) was commonest organism after Staphylococcus aureus (221, 32.2%) among Gram positive and Pseudomonas was the commonest (139, 47.1%) among Gram negative. Lowest MIC noted for moxifloxacin (0.08 µg/ml) and cefazolin (0.1µg/ml) in Staphylococcus group, Ciprofloxacin (0.008µg/ml) in Gram negative bacilli.14 Gram positive bacteria and 32 Gram negative bacteria isolated from endophthalmitis were found resistant against antibiotics. Last five years resistance pattern is comparatively higher.

Conclusions:

Incidence of drug resistance is uncommon amongst the bacterial isolates obtained from infective keratitis and commensals in conjunctival cul de sac. However, resistant strains are more common in last five years compare to the first years in isolates obtained from cases of postoperative endophthalmitis.

Financial Disclosure:

None