Novel expression markers and their epigenetic regulation as a translational therapeutic application in corneal scar

Session Details

Session Title: SMILE vs PRK, Ocular Surface Disease

Session Date/Time: Monday 24/09/2018 | 08:00-10:30

Paper Time: 09:58

Venue: Room A3, Podium 3

First Author: : R.Dalal INDIA

Co Author(s): :    R. Shetty   A. Ghosh   P. Khamar   M. Rajan           

Abstract Details

Purpose:

Study molecular factors and effect of prophylactic application of epigenetic modulation on scarring in photorefractive keratectomy (PRK).

Setting:

Narayana Nethralaya, Bangalore, India University of Missouri, Columbia, Missouri, USA

Methods:

Corneal epithelium samples collected intra- op were studied for gene expression profile from 6 corneal haze eyes. Fibrosis was studied in vitro using differential doses of TGFβ on cultured corneal epithelial cells. Rabbit PRK model was used to study drugs to treat post PRK corneal scarring.

Results:

WNT pathway and PREX1(oxidative stress marker) levels were altered in patient epithelium with haze and could modulate wound healing in vitro. Pro-fibrotic expression was altered by histone deacetylase (HDAC) inhibitor SAHA. Rabbit PRK eyes show significant reduction in postoperative haze without keratocyte cell death when SAHA was applied.

Conclusions:

Novel genes are involved in pathogenesis of haze post PRK. Modulation of haze predisposing pathways may be mediated by HDAC modulators to prevent haze post PRK in future.

Financial Disclosure:

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