Atomic force microscopy analysis of the corneal stromal roughness after LASIK flap cut using different femtosecond lasers: Intralase vs Visumax vs LenSx

Session Details

Session Title: LASIK II

Session Date/Time: Tuesday 25/09/2018 | 16:30-18:00

Paper Time: 16:54

Venue: Room A3, Podium 2

First Author: : J.Gros-Otero SPAIN

Co Author(s): :    S. Ketabi   S. Casado   R. Cañones-Zafra   C. Villa-Collar   G. Bernabau-Arias   M. Teus     

Abstract Details

Purpose:

To compare the induced corneal stromal roughness after femtosecond laser LASIK flap cut in porcine eyes, measured with an atomic force microscopy

Setting:

Novovisión, Clínica Rementería, HM Vallés, IMDEA Nanociencia, Madrid, Spain

Methods:

We designed an experimental study that included 9 enucleated porcine eyes, 3 eyes for each Femtosecond laser: Intralase, Visumax, and LenSx. The standard Femtolaser parameters were used. After a LASIK flap was cut, we measured the corneal stromal bed roughness with a JPK NanoWizard II atomic force microscopy in contact mode, using Olympus OMCL-RC800PSA commercial silicon nitride cantilever tips. Surface measurements were made on 10 areas of 20μm x 20μm of the central stromal bed at 512x512 point resolution. We evaluated surface roughness using the root-mean-square deviation from a perfectly flat surface value, within the analyzed area.

Results:

The corneal stromal bed roughness (evaluated by the RMS deviation form a perfectly flat surface) was: 230100 nm for Visumax, 370100 nm for LenSx and 360120 nm for Intralase. The smoothest stromal bed surface after a femtosecond laser cut was obtained with the Visumax Laser, that was statistically different from the other groups (p<0,05).

Conclusions:

The stromal bed roughness after a femtosecond laser cut is different depending on the device studied. Visumax is the one that obtains the smoothest surface among the lasers analyzed in this study.

Financial Disclosure:

... research is funded, fully or partially, by a competing company