Preparation and characterisation of a novel cross-linked collagen: gelatin hydrogel for its potential use as corneal engineering

Session Details

Session Title: Cross-Linking

Session Date/Time: Tuesday 25/09/2018 | 14:00-16:00

Paper Time: 14:00

Venue: Room A3, Podium 3

First Author: : H.Aghamollaei IRAN

Co Author(s): :    K. Jadidi   H. Goodarzi   F. Nejat              

Abstract Details

Purpose:

The aim of this study was to construction and investigation of biocompatibility, transparency, and physical properties of a tissue-engineered collagen-gelatin (Col-Gel) hydrogel bioscaffold for corneal regeneration.

Setting:

Baqiyatallah University of medical sciences, Tehran, Iran

Methods:

The scaffold is fabricated from type I collagen and gelatin (the mass ratio of Col: Gel=1:10) cross-linked with N-(3-Dimethylaminopropyl)-N'-ethyl carbodiimide hydrochloride and N- hydroxysuccinimide. Mechanical properties, transparency, hydrophilicity, and biocompatibility were analyzed by appropriate methods such as compressive modulus, Surface contact angle, MTT, spectrophotometry and scanning electron microscopy (SEM). For cell experiment, human mesenchymal stem cells, as keratocyte progenitor, were cultured onto the surface of hydrogel and the response of stem cell to the scaffold was investigated by hematoxylin-eosin staining and SEM.

Results:

Results showed that this bioengineered hydrogel has adequate mechanical properties. Optical transparency of this scaffold is similar to the native cornea. The viability of the cells assayed by MTT confirmed that crosslinked Col-Gel scaffold is biocompatible and not cytotoxic. Histological examination indicated that morphological appearances of the cells are normal. SEM images showed that human mesenchymal stem cells have been attached and infiltrated to hydrogel through interconnected pores.

Conclusions:

Due to appropriate hydrophilicity, optical transparency, biocompatibility and mechanical properties, this Col- Gel scaffold is an appropriate candidate for corneal tissue engineering.

Financial Disclosure:

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