Pharmacokinetics of caffeine in the lens capsule/epithelium after peroral intake: a pilot randomised controlled study

Session Details

Session Title: Paediatric Ophthalmology & Other Topics

Session Date/Time: Tuesday 25/09/2018 | 08:00-10:30

Paper Time: 09:30

Venue: Room A3, Podium 2

First Author: : M.Kronschläger AUSTRIA

Co Author(s): :                        

Abstract Details

Purpose:

To determine the pharmacokinetics of perorally administered caffeine, a widely consumed and potent dietary antioxidant, in the anterior lens capsule and lens epithelial cells, a crucial cell monolayer for cataract development.

Setting:

Hanusch Hospital, Vienna, Austria

Methods:

Bilateral cataract patients were scheduled for cataract surgery with a caffeine abstinence of 1 week before surgery of each eye. At the day of surgery of the second eye patients were administered no drink (0 mg group) or coffee with 60 mg caffeine, 120 mg caffeine or 180 mg caffeine. After capsulorhexis the lens capsule including lens epithelial cells was transferred to a test tube for analysis of caffeine concentration by gas chromatography-mass spectrometry (GC – MS/MS).

Results:

Coffee consumption significantly (p < 0.05) increased caffeine levels of the lens capsule/epithelium in the 60 mg, 120 mg and 180 mg group. Caffeine concentrations (caffeine ng/lens capsule/epithelium) measured as difference between 1st and 2nd eye were -0.52 ± 1.16 (0 mg group, n = 7), 1.88 ± 2.02 (60 mg group, n = 8), 2.09 ± 0.67 (120 mg group, n = 9) and 3.68 ± 1.86 (180 mg group, n = 9). The increase constant of caffeine in a linear regression model was estimated as a 95% CI 0.02 ± 0.0046 (d.f. 25; r = 0.85).

Conclusions:

Peroral intake of coffee significantly increased caffeine concentrations in the lens capsule and lens epithelial cells in a dose-dependent manner. This information is important for further investigations on preventing cataract.

Financial Disclosure:

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