Quantification of growth factors and fibronectin in different preparations of eye platelet-rich plasma (E-PRP)

Session Details

Session Title: Ocular Surface & Quality of Vision

Session Date/Time: Monday 24/09/2018 | 14:00-16:00

Paper Time: 14:24

Venue: Room A3, Podium 1

First Author: : A.Rodriguez SPAIN

Co Author(s): :    J. Alio                    

Abstract Details

Purpose:

Autologous platelet-rich plasma (E-PRP) is a blood derived product reporting successful results in ophthalmology. The purpose of this study is to quantify the presence of growth factors in E-PRP comparing their concentration when applying four different treatments.

Setting:

This investigational study was carried out in the Research & Development Laboratory of Vissum Alicante.

Methods:

To prepare E-PRP as a topical eye drop, blood from different healthy volunteers was used. After the preparation, the final plasma product was aliquoted for further implementation of the four treatments including fresh E-PRP, frozen E-PRP at -20ºC for 3 months, fresh-spin, and frozen-spin at -20ºC. The concentration of the growth factors PDGF-BB, TGF-β1, EGF, VEGF-A and fibronectin involved in wound healing was determined. Posterior statistical analysis was performed in order to establish significant differences between groups and between growth factors in relation with the amounts of platelets.

Results:

The used methodology for obtaining the E-PRP was the optimum because platelets doubled in number. The presence of red blood cells in the E-PRP is almost non-existent which makes it suitable for ocular topical application and it has a very small number of leukocytes. The concentration of PDGF-BB, and EGF was significantly higher (p<0,01) when the samples were frozen at -20ºC, however no significant differences were observed among the treatments for TGF-β1, VEGF-A and fibronectin (p>0,05). The concentration of growth factors in the E-PRP does not necessarily correlate with the number of platelets/μl.

Conclusions:

Our results conclude that freezing the E-PRP will increase the concentration of important proteins taking place in wound healing maintaining the biological activity in this successful treatment involved in corneal surface tissue regeneration.

Financial Disclosure:

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