Posters

A preliminary study on the establishment of an animal model of conjunctivochalasis

Poster Details

First Author: Q.Li CHINA

Co Author(s):                        

Abstract Details

Purpose:

To explore a feasible method on the establishment of an animal model of conjunctivochalasis.

Setting:

Conjunctivochalasis (CCH) is presented for more than 70y, but scientists still haven’t found a recognized animal model.

Methods:

Twelve clean-grade New Zealand white rabbits were divided into four groups (n=3/group): the control group (one received no interventions, and the others underwent subconjunctival injection of sterile water), the matrix metalloproteinase (MMPs) group (administered subconjunctival injection of matrix metalloproteinase-3), the aging group (administered subcutaneous injection of D-galactose), the tumor necrosis factor-α (TNF-α) solution group (administered eye drops of tumor necrosis factor-α). Anterior segment photography, conjunctival tissue light microscopy and transmission electron microscopy were performed after 12wk.

Results:

Among all groups, the MMPs group had the following changes: the looser connection between the inferior bulbar conjunctiva and sclera; the more disordered collagen fibers (Trichrome staining) and the broken elastic fibers (Aldehyde-fuchsin staining); the focal necrosis of fibroblasts (transmission electron microscopy).

Conclusions:

Administration of MMPs may be a feasible method for the establishment of an animal model of conjunctivochalasis.

Financial Disclosure:

None