Corneal endothelial cell viability to impurities in trypan blue vital staining dye

Session Details

Session Title: Presented Poster Session: Medical Cornea

Venue: Poster Village: Pod 3

First Author: : S.Srinivasan UK

Co Author(s): :    J. Mehta   G. Peh Swee Lim                    

Abstract Details

Purpose:

To evaluate the level of impurities in various commercially available trypan blue vital eye and to assess the corneal endothelial cell viability to the impurities in trypan blue vital staining dye.

Setting:

University Hospital Ayr, Ayr, Scotland, UK Singapore Eye Research Institute, Singapore

Methods:

High performance liquid chromatography was performed to evaluate and quantify the levels of impurities in different commercially available trypan blue staining dye. Primary human corneal endothelial cells obtained from eye bank corneas were expanded in lab to produce endothelial cell lines using the dual media approach. These cells lines were exposed to different known concentrations and impurities of trypan blue from different manufacturers. Flow cytometric analysis and cell impendence were analysed to assess corneal endothelial cell viability.

Results:

Corneal endothelial cell viability varied depending on the concentration and impurities present in various trypan blue stains. Human corneal endothelial cells seem to appear more viable in trypan blue stains that have less impurities and low concentration.

Conclusions:

Commercially available trypan blue stains show varying levels of concentrations and impurities. High levels of impurities in these stains may effect human corneal endothelial cell viability.

Financial Disclosure:

None