A changing pattern in the last 10 years of in vitro antimicrobial susceptibility of bacterial keratitis isolates, endophthalmitis and normal ocular commensals from Eastern India

Session Details

Session Title: Ocular Infections & Other Topics

Session Date/Time: Sunday 15/09/2019 | 08:00-10:00

Paper Time: 08:54

Venue: Free Paper Forum: Podium 4

First Author: : S.Saha INDIA

Co Author(s): :    J. Sengupta   A. Khetan                          

Abstract Details

Purpose:

To report the changing antibiotic susceptibility profile of bacterial keratitis isolates and commensal microbial flora of conjunctival cul de sac isolated over the last 10 years (2008-13 & 2014-18).

Setting:

Tertiary care eye hospital of eastern India

Methods:

Laboratory records of all consecutive cases of bacterial corneal ulcers and normal commensals were retrospectively reviewed. Data noted included microorganism isolated and antibiotic culture sensitivity to the fourth generation fluoroquinolones (moxifloxacin, gatifloxacin), fluoroquinolones (ofloxacin and ciprofloxacin), cefazolin, and to the aminoglycosides (tobramycin and gentamicin) was performed using by the Kirby-Bauer disc diffusion method.

Results:

Total 980 bacterial isolates identified from corneal ulcer (618 cases), postoperative endophthalmitis (175 cases) and conjunctival swab culture (187 cases). Of these, 685 (70%) were Gram-positive and 295 (30%) were Gram-negative. Staphylococcus epidermidis (327, 47.7%) was most common followed by Staphylococcus aureus (221, 32.2%) among gram positive and Pseudomonas was commonest (139, 47.1%) among gram negative. Lowest MIC was noted for moxifloxacin (0.08 µg/ml) in Staphylococcus group, Ciprofloxacin (0.008µg/ml) in Gram negative bacilli. 14 Gram positive bacteria and 32 Gram negative bacteria from postoperative endophthalmitis were found resistant to all mentioned antibiotics. Last five years resistance pattern is higher.

Conclusions:

Incidence of drug resistance is remarkable amongst the bacterial isolates obtained from infective keratitis and commensals in conjunctival cul de sac. However, resistant strains are more common in last five years compare to the first years in isolates obtained from cases of postoperative endophthalmitis.

Financial Disclosure:

None