Posters

Comparison of adhesive strength and biocompatibility among suture, fibrin glue and LAMBA in amniotic membrane transplantation

Poster Details

First Author: W.Park KOREA

Co Author(s): J. Min   L. Jin   T. Park   S. Maeng   H. Cha        

Abstract Details

Purpose:

We compared adhesive strength and histological findings among suture, fibrin glue and light-activated mussel protein-based bioadhesive (LAMBA) in amniotic membrane transplantation to figure out biocompatibility of LAMBA and applicability in clinical field.

Setting:

There is no financial or conflict of interest to disclose.

Methods:

48 of rabbit were used. 7x10 mm conjunctiva defect was made on both side of SR and amniotic membrane (AM) was transplanted. Rabbits were grouped by the method of AM transplantation in group A (suture), group B (fibrin glue), and group C (LAMBA). The adhesive strength was measured at 1 hour, 3 days and 10 days with digital push pull gauge. Tissues were taken at 3 days, 10 days, and 1 month. Inflammatory cells were counted and length of AM attached on sclera was measured.

Results:

At 1 hour after AM transplantation, adhesive strength of group A and C were higher than group B, In H & E stain, inflammation cells of group A and C were more than group B after 3 day. In length of AM attached on sclera, group A and C were longer than group B after 3 days. In Masson trichrome stain, group C shows lower fibrosis than group A and B.

Conclusions:

AM transplantation using LAMBA has stronger adhesive strength and the longer length of attached AM on sclera, but has more severe inflammation than that of fibrin glue. When protein concentration of LAMBA dilutes for diminish the inflammation and maintains the proper adhesion strength, LAMBA can substitute suture or fibrin glue on AM transplantation.

Financial Disclosure:

None