Posters

Atomic force microscopy analysis of the surface roughness changes in a standard hydrophobic IOL (Acrysof ®) before and after full hydration

Poster Details

First Author: A.Katsanos GREECE

Co Author(s): J. Gros-Otero   S. Ketabi   C. Villa-Collar   R. Ca�ones-Zafra   M. Garcia-Gonzalez   M. Teus     

Abstract Details

Purpose:

To measure with an atomic force microscopy (AFM) the surface roughness changes in a standard hydrophobic intraocular lens (IOL) (Acrysof®, Alcon SN60WF) prior and after full hydration.

Setting:

Clínica Novovisión, Madrid, Spain. Clínica Rementería, Madrid, Spain. Universidad Europea de Madrid, Madrid, Spain. Príncipe de Asturias Hospital, University of Alcalá, Alcalá de Henares, Madrid, Spain. University of Ioannina, Ioannina, Greece.

Methods:

Experimental study to measure the changes in surface roughness of an IOL after hydration in order to obtain a standard experimental model to measure IOL surface roughness.An Acrysof IOL was removed from the container in ambient air,placed on a pristine glass coverslip,and its surface roughness was measured using the JPK NanoWizard II® AFM, in “contact mode”. AFM scanning of areas ranging 50x50 μm², 10x10 μm² and 2x2 μm² were performed at 512x512 point resolution.Subsequently, the same IOL was submerged in freshly extracted milli-Q water and inspected in liquid.Regions very close to those ones previously selected in air were chosen again

Results:

The roughness (evaluated with the root-mean-square value) of the IOL optical area were 0.96 nm in dry conditions versus 1.84 nm after inmersion in mili-Q water at 2x2 μm²; 4.20 nm versus 5.56 nm at 10x10 μm² and 5.31 nm versus 6.97 nm at 50x50μm². These differences were statistically significant (P <0.05).

Conclusions:

Hydration conditions may alter the surface roughness measurements in hydrophobic IOLs. We recommend AFM roughness measurement after full hydration of the IOL.

Financial Disclosure:

None