Optisol supplemented with 0.255 µg/ml Amphotericin B does not eliminate yeast at 5˚C

Session Details

Session Title: Cornea & Miscellaneous
Session Date/Time: Friday 15/02/2019 | 10:30-12:30
Paper Time: 11:54
Venue: Room MC3

First Author: R.Kowalski USA
Co Author(s):                  

Abstract Details

Purpose:

Fungal infections in lamellar keratoplasty is a growing concern. Optisol-GS does not contain an antifungal and supplementation with 0.255 µg/ml has been considered. This study tests the ability of 0.255 µg/ml Amphotericin B (AmpB) in Optisol-GS to eliminate yeast contamination of corneal tissue at 5 degrees C.

Setting:

Laboratory Study - University of Pittsburgh, Ophthalmic Microbiology

Methods:

Five isolates of Candida species were tested in Optisol with and without AmpB. Corneal rims were inoculated with 4 respective loads: 0, 10, 100, and 1000 cfu. One set was filled with Optisol plus AmpB and the other with Optisol only. All plates were incubated at 5˚C for 48 hours. After 48 hours, all corneal rims were placed into 10 ml of yeast extract peptone dextrose medium (YPD); a swab culture of each well was plated onto Sabouraud plates; and all plates with the remaining Optisol were incubated at 30˚C. MICs and MBCs were determined.

Results:

All corneoscleral specimens were positive regardless of fungal load or presence of AmpB. All controls remained negative. MICs and MBCs were equivalent and ranged between 0.5 to 2.0 µg/ml.

Conclusions:

Amphotericin B at a concentration of 0.255 µg/ml in Optisol-GS at 5˚C over 48 hours does not sterilize corneal specimens contaminated with yeast.

Financial Disclosure:

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