First Author: R.Liegl GERMANY
Co Author(s): C. Wertheimer M. Kernt A. Kampik D. Docheva K. Eibl Lindner
Purpose:
To evaluate the effect of alkylphosphocholines (APCs) on human lens epithelial cell (LEC) migration with Time Lapse imaging, a novel method in ocular cell migration evaluation.
Setting:
Department of Ophthalmology, Ludwig-Maximilians-University Munich, Germany
Methods:
Human lens epithelial cells were seeded in 6 well plates and exposed to Alkylphosphocholines in concentrations of 0.1µM and 1.0µM, untreated cells served as controls. Cells were allowed to adhere for four hours and then put into a Time Lapse chamber. Photographs were continuously taken for 12 hours. The videos were then analyzed in respect of cell migration distance and cell velocity using Zeiss AxioVision LE, ImageJ and MtrackJ software.
Results:
Alkylphosphocholines are effective inhibitors of human lens cell migration in non toxic concentrations as observed in Time Lapse imaging. Concentrations of up to 1mM showed no significant reduction in cell viability as tested by the MTT test and a Live/Dead assay. Cell migration was reduced to 69% in a concentration of 0.1µM and to 65% in a concentration of 1µM compared to untreated cells in terms of distance. Mean velocity was reduced to 60.2% at a concentration of 0.1µM and to 56.7% at 1.0µM compared to controls.
Conclusions:
Posterior capsule opacification (PCO) is still a common issue after successful cataract surgery. One major property in the formation of PCO is the ability of human lens epithelial cells to migrate. In this study we could not only demonstrate that APC“s are capable of inhibiting LEC migration in vitro in non toxic concentrations but also that Time Lapse imaging is a sophisticated method to evaluate cell migration and cell velocity in vitro. FINANCIAL DISCLOSURE?: No
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