First Author: S.Chiang TAIWAN
Co Author(s): M. Tsai H. Shui
Purpose:
The purpose of this study was to compare protein profiles of aqueous humor (AH) from white cataract patients with that from control cataract patients who has no white-cataract characteristics, in order to give an insight into underlying mechanisms of development of white cataract.
Setting:
AH samples were collected during surgery from both white cataract group (7 cases) and mild cataract group (17 cases), with matching age and gender between the two groups.
Methods:
Total AH protein concentration was measured uring Bradford method. AH proteins were then separated by two-dimensional polyacrylamide gel electrophoresis (2-DE). Protein spots on 2-DE gels were visualized by SyproRuby, and their expressional profiles were analyzed by a 2-DE software. Differentially expressed proteins on the gels beween the two groups were excised, digested with trypsin, and identified by peptide mass fingerprintig using MALDI-TOF mass spectrometry.
Results:
Concentrations of total AH protein were significantly higher in patients with white cataract than those in control subjects (Mann-Whitney U test, p = 0.003). Four spots showing significant difference in protein abundance in AH between the two groups were successfully identified, including neural cell adhesion molecule L1-like protein, integrin beta-3, Rab GDP dissociation inhibitor beta, and spermine synthase.
Conclusions:
The difference of protein profiles in AH can be used to differentiate white cataract from other cataract conditions. Importantly, the identified proteins could serve as biomarkers for predicting the development and understanding the mechanisms of white cataract. FINANCIAL DISCLOSURE?: No
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