First Author: J.Lee SOUTH KOREA
Co Author(s): B. Oum N. Kim H. Jeon
Purpose:
To evaluate the biologic effects of fluoroquinolone eye solutions on cultured human corneal epithelial cells in vitro.
Setting:
Department of Ophthalmology, School of medicine, Pusan National University, Pusan, Korea
Methods:
The efficacies of the fluoroquinolone eye solutions Tarivid®, Cravit®, Ozex®, Vigamox® and Gatiflo® were evaluated using the MTT assay. Cellular damage was determined using the lactate dehyrogenase (LDH) assay. Cellular proliferation was determined using a migration and wound healing assay. Cellular morphologies were examined by performing inverted light microscopy and transmission electron microscopy.
Results:
The metabolic activity of the corneal epithelial cells decreased and the LDH titer increased with longer exposure times to all the fluoroquinolone eye solutions. Especially, the LDH titers significantly increased after exposure to the 4th generation fluoroquinolones such as Vigamox® and Gatiflo®. Cellular migration was not observed after exposure to Ozex®, Vigamox®, and Gatiflo®. Severe cellular morphological damage was observed on inverted light microscopy and transmission electron microscopy after exposure to the 4th generation fluoroquinolones.
Conclusions:
Among several generations of topical fluoroquinolone eye solutions, the 4th generation fluoroquinolones induced severer damage to the cultured human corneal epithelial cells than did the 2nd and 3rd generation fluoroquinolones. FINANCIAL DISCLOSURE?: No
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