Posters

Histopathology of silicone oil keratopathy

Poster Details

First Author: D. Ozek TURKEY

Co Author(s):    O. Evren Kemer                    

Abstract Details

Purpose:

Evaluation of histopathological findings of corneal buttons after penetrating keratoplasty (PK) in silicone oil related keratopathy patients.

Setting:

Ankara Numune Training and Research Hospital, Ophthalmology Clinic, Ankara Ankara Numune Training and Research Hospital, Pathology Clinic, Ankara

Methods:

Corneal buttons of 14 eyes, extracted between 2012 – 2015 due to silicone keratopathy, were examined histopathologically. Eleven patients were male, 3 were female with mean age of 38.5 (9 – 76) years. Traumatic retinal detachment accompanied corneal opacity in all patients. Nine eyes had multiple surgical operations. For histological evaluation, corneal buttons were cut 3 µm and sections were stained with haematoxylin and eosin first for routine evaluation. Then several sections were also stained with Periodic acid–Schiff (PAS) and trichome stains to examine different layers of cornea.

Results:

The most common finding on histopathological examination were collegenous stromal edema and detachment of collagen fibers. Depending on silicone oil exposure duration, hyalinization rather than stromal edema was observed on some tissues. Thickening of Bowman’s layer and irregularity together with vascular proliferation behind Bowman’s layer were observed. Stromal edema associated cracks were observed on Descemet’s membrane. Corneal epithelial layer also showed several changes like: detachment from basement membrane and acantholytic changes. These changes are signs of intracellular edema in epithelial cells.

Conclusions:

Silicon oil exposure to corneal results in histopathological changes at all corneal layers. Most prominent feature was stromal thickening which turns out to hyalinization with increased silicon exposure time.

Financial Disclosure:

NONE