Comparison of central corneal thickness and endothelial cell measurements by Pentacam Schiempflug camera system and two noncontact specular microscopes

Session Details

Session Title: Cornea Medical II

Session Date/Time: Tuesday 13/09/2016 | 08:00-10:30

Paper Time: 10:06

Venue: Hall C3

First Author: : I.Karaca TURKEY

Co Author(s): :    S. Guven Yilmaz   M. Palamar Onay   H. Ates              

Abstract Details

Purpose:

To investigate the correlation of central corneal thickness (CCT) measurements between Pentacam Schiempflug camera system and two noncontact specular microscopes, and corneal endothelial cell evaluation between two noncontact specular microscopes.

Setting:

Ege University, Department of Ophthalmology, Izmir,Turkey

Methods:

A total of 100 eyes of 50 healthy subjects with the mean age of 36.74±8.59 (range 22-57) years were examined by Pentacam Schiempflug Analyzer and two noncontact specular microscopes (NIDEK CEM-530, KONAN CellChek XL). Measurement differences and agreement between instruments were determined by Intraclass correlation analysis.

Results:

CCTs were correlated in between all devices, with having CellChek XL the thickest and CEM-530 the thinnest measurements (ICC=0.83; p=0.000). Mean endothelial cell density (ECD) with CEM-530 was lower than CellChek XL (2613.17±228.62 and 2862.72±170.42 cells/mm2 respectively; ICC=0.43; p=0.000). Mean coefficient of variation (CV) was 28.57±3.61 in CEM-530 and 30.30±3.53 in CellChek XL (ICC=0.23; p=0.005). Hexagonality (HEX) with CEM-530 was higher than with CellChek XL (68.70±4.16% and 45.19±6.58%, respectively (ICC=0.009; p=0.180)). The mean of the differences (CellChek XL minus CEM-530) for ECD was (249.55±142.19 cells/mm2 (95% CI 221.34–277.76); 1.71±4.36% (95% CI 0.85–2.57) in CV; and -23.51±7.42 for percentage of hexagonal cells.

Conclusions:

Values for ECD between CellChek XL and CEM-530 correlate well, but the ECDs calculated by CellChek XL are higher than CEM-530 values. Both HEX and CV vary greatly and do not correlate sufficiently. Thus we recommend not to use CellChek XL and CEM-530 instruments interchangeably. Both CEM-530 and CellChek XL specular microscopy instruments are reliable devices for CCT measurements.

Financial Disclosure:

NONE