Characterization of platelet-rich plasma (E-PRP): quantification of growth factors and fibronectin

Session Details

Session Title: Cornea Medical II

Session Date/Time: Tuesday 13/09/2016 | 08:00-10:30

Paper Time: 08:54

Venue: Hall C3

First Author: : M.Tiveron Jr SPAIN

Co Author(s): :    A. Rodriguez   S. Gisbert   J. Alio              

Abstract Details

Purpose:

Autologous platelet-rich plasma (E-PRP) is a blood derived product reporting successful results in ophthalmology, attributed to the growth factors synthesized and present in blood. The purpose of this study is to quantify the presence of growth factors and fibronectin in platelet-rich plasma comparing their concentration when applying four different treatments.

Setting:

Vissum Alicante Spain

Methods:

To prepare platelet-rich plasma (E-PRP) as topical eye drop, blood from six different healthy patients was used. After a centrifugation the final plasma product was aliquoted for further implementation of the four treatments including fresh E-PRP at 4-8ºC, frozen E-PRP at -20ºC for 3 months, fresh-spin at 4-8ºC, and frozen-spin at -20ºC. The concentration of several growth factors (PDGF-BB, TGF-β1, EGF, VEGF-A) and fibronectin involved in wound healing was determined and posterior statistical analysis was performed in order to establish significant differences between growth factors and between groups in relation with the amounts of platelets.

Results:

All of the six patients of the study came out as healthy, without dry eye, in the OSDI test. It was proved that the methodology for obtaining the E-PRP was the optimum because platelets doubled in number. The concentration of PDGF-BB, TGF-β1 and EGF was significantly higher (p<0,05) when the samples were frozen at -20ºC, however no significant differences were observed among the treatments for VEGF-A and fibronectin. It is shown that there is a positive correlation between the number of platelets and the secretion of growth factors for fresh E-PRP.

Conclusions:

Our results conclude that freezing the E-PRP will increase the concentration of important proteins taking place in wound healing maintaining the biological activity in this successful treatment involved in corneal surface tissue regeneration.

Financial Disclosure:

NONE