Post-LASIK ectasia and progressive keratoconus: understanding the mechanistic pathway beyond topography

Session Details

Session Title: Investigations and Complications Management

Session Date/Time: Tuesday 13/09/2016 | 13:30-15:15

Paper Time: 14:16

Venue: Hall C3

First Author: : N.Pahuja INDIA

Co Author(s): :    R. Shetty   A. Agrawal   R. Deshmukh   R. Shroff   A. Ghosh   S. Sethu     

Abstract Details

Purpose:

To study the association between tear inflammatory biomarkers and ectasia

Setting:

Tertiary eye hospital, Bangalore.

Methods:

Post-LASIK ectasia (n=6) and age matched controls (n=6) were included in a cross-sectional study. Tears were collected on Schirmer’s strips were analysed in cell biology lab. Levels of 22 tear inflammatory mediators (IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, MCP1, MIP1β, RANTES, ICAM1, IFNα) were measured using cytometric bead array and were correlated to the clinical parameters. Similarly, Inflammatory mediators were analyzed in progressive keratoconus (n=9) and grade matched stable keratoconus (n=9)

Results:

Post-LASIK ectasia: Inflammatory mediators were observed to be higher in the tears of patients with post-LASIK ectasia. Furthermore, ≥ 2 fold higher levels of most of these mediators (IL-1α, IL-2, IL-4, IL-8, MCP1, MIP1β, RANTES and IFNα) were observed as compared to controls. Keratoconus: Tear inflammatory mediators were observed to be increased in the tears of patients with keratoconus. A fold difference of ≥ 2 was observed in the levels of tear IL-1α, IL-1β, IL-4, IL-23, IL-17A, IL-17F, IFNγ, MCP1 and RANTES.

Conclusions:

An increase in the inflammatory mediators was observed in both post-LASIK ectasia and keratoconus. In addition, a distinct tear mediators profile was observed between post-LASIK ectasia and keratoconus which can be novel targets to possibly manage iatrogenic ectasia and/or delay or halt progression of keratoconus.

Financial Disclosure:

NONE