Posters

Explantation of corneal inlays for presbyopia correction in an experimental study in vivo

Poster Details

First Author: I.Mushkova RUSSIA

Co Author(s): Y. Shkandina                    

Abstract Details

Purpose:

To present changes of corneal tissue using optical coherent tomography (OCT), confocal microscopy (CM) and light microscopy (LM) after explantation developed corneal inlays made of polymer materials based on hydroxyethylmethacrylate (HEMA) and olygourethanmethacrylate (OUMA)

Setting:

S. Fyodorov Eye Microsurgery Federal State Institution, Moscow, Russia

Methods:

Eight 50-microns thickness corneal inlays were implanted to right eyes of eight rabbits, while left eyes remained intact.Group 1 included 4 right eyes with inlays made of HEMA,Group 2 included 4 right eyes with 4 inlays made of OUMA.Control group included 8 left eyes.We performed manually the same corneal pocket(CP) in both eyes. Left eyes (control group) were not subjected to implantation. In three months all experimental animals were underwent explantation of corneal implants. Corneas of both eyes of all animals by OCT, CM and LM were analyzed in two months after explantation in order to reveal post-implantation changes

Results:

OCT:the demarcation line,the base of CP were not found in all groups,perhaps because of the accelerated healing of rabbit cornea.CM:we revealed pseudo keratinization of corneal epithelium,in the early explanted inlay area pseudocellular membrane was formed,it was denser in Group 1 than in Group 2.In the environment of the membrane we revealed a decline of keratocytes and lack of innervation.The middle layers of the corneal stroma were folded and hypocellular.The posterior epithelium was without pathological changes.LM:Groups1,2-epithelium was thickened at the base of CP,in the middle of stroma a discharge line was established(previous CP),endothelium remained without changes.In group3 changes were not revealed

Conclusions:

After inlay explantation by confocal microscopy, a pseudocellular membrane was visualized in stroma of the cornea by confocal microscopy in the previously placed implant zone. This fact refutes the absolute reversibility of the intervention. However, during biomicroscopic examination, OCT and light microscopy of the rabbit cornea, significant pathological changes were not detected, it makes necessary to perform further study of the developed inlays on the model of the human cornea

Financial Disclosure:

None