Posters
Experimental scleral study after cross-linking exposure
Poster Details
First Author: A.Milingert RUSSIA
Co Author(s): S. Borzenok T. Sokolovskaya
Abstract Details
Purpose:
to determine morphological and bio-mechanical properties of scleral tissue after a cross-linking exposure in individuals with different types of axial refraction of the eye.
Setting:
The S. Fyodorov Eye Microsurgery Federal State Institution, Moscow, Russia
Methods:
the study included 16 isolated scleral fragments of 16 human cadaver eyes. The anterior-posterior axis (APA) of the studied eyes was 23.56�Â�±3.06mm (20.05-26.62). Samples of sclera (5x5mm) were cut out with a microsurgical blade from the posterior pole of the eye, and then were exposed to a cross-linking effect. Further, all the fragments of the sclera were cultivated in a nutrient medium. Biomechanical tests of samples of scleral tissue after the cross-linking exposure was performed using the universal testing Instron-3322 machine. Then the samples of scleral tissue was investigated using the raster ion-electron microscope Quanta 200 3D.
Results:
An increase in strength indices and Young's Modulus were noted in all tissue samples, after cross-linking within the study of biomechanical properties of fragments of scleral of posterior pole of the eye.The strength indices of scleral tissue after the crosslinking exposure increased significantly (p<0.05) from 13.4�Â�±1.3 to 20.2�Â�±1.2Mpa in eyes with a hyperopic type, from 9.8�Â�±1.4 and 14.7�Â�±1.3 Mpa �â�� with a myopic type, from 12.2�Â�±1.3 to 19.8�Â�±1.5 MPa �â�� with emmetropia. Morphological investigations of the sclera showed decreasing the distance between the bundles of collagen fibrils and the formation new of adhesive collagen structures in its superficial layers.
Conclusions:
Crosslinking leads to pronounced morphological changes of the sclera, that is accompanied by an in crease of the strength characteristics of the tissue.
2. It is possible to develop a method of the impact on the fibrous tunic of the eye in open-angle glaucoma to stabilize the course of disease modeling the biomechanical properties in the most predictably dangerous areas
Financial Disclosure:
NONE
