Gene expression of TGF beta isoforms and its receptors in parts of trabecular meshwork in patients with glaucoma
Session Details
Session Title: Presented Poster Session: Glaucoma and Imaging
Venue: Poster Village: Pod 3
First Author: : W.Maruszczyk POLAND
Co Author(s): : M. Kimsa M. Machalska S. Sapeta M. Dorecka U. Mazurek E. Mrukwa-Kominek
Abstract Details
Purpose:
Glaucoma is chronic ophthalmology disease witch cause irreversible optic disc and retinal ganglion cells damage occurring as a visual field lost. The pathogenesis of this condition is still uncertain. Nowadays, the obstruction on trabecular meshwork level is one of the most potent cause of this disease, in witch transforming growth factor beta (TGFβ) may play a role.
The aim of this study is to evaluate the gene expression of isoforms of TGFβ and its receptors, at the mRNA level, in the removed parts of the iridocorneal angle (trabeculum) in patients with glaucoma who underwent trabeculectomy with basal iridectomy.
Setting:
Department of Ophthalmology, Prof. K. Gibiński University Clinical Centre, School of Medicine in Katowice, Medical University of Silesia in Katowice, Katowice - Poland.
Study was performed due to Medical University of Silesia in Katowice grants No KNW-1-190/N/4/0 and KNW-2-047/D/4/N
Methods:
The study group included 19 eyes qualified for glaucoma surgery, trabeculectomy with basal iridectomy. Diagnostic material, collected from each patient, included a part of the iridocorneal angle. Tissues were collected into separate tubes with buffer to prevent the degradation of RNA (TRIzol), and then stored at -20stC until further assays. Total RNA extraction was performed using TRIzol reagent according to the manufacturer's instructions. QRT-PCR reaction was performed (TGFβ1, TGFβ2, TGFβ3, TGFβ1R, TGFβ2R, TGFβ3R and 2 endogenous controls - GAPDH and β-actin. PAGE and fixing of the melting temperature confirmed the specificity of the PCR reactions.
Results:
There were statistically significant differences in the gene expression of TGFβ isoforms - TGFβ1: 17418,45; TGFβ2: 6041,48; TGFβ3: 7240,11 [mRNA copies / ug of RNA] (p =0,0035). There were no statistically significant differences in the gene expression of TGFβ receptors - TGFβ1R: 11388,42; TGFβ2R: 13152,66; TGFβ3R: 5645,16 [mRNA copies / ug of RNA] (p>0,05).
Conclusions:
The study confirmed the presence of TGF beta isoforms and its receptors in the trabeculum in glaucoma patients. Some differences in the expression of the TGF beta isoforms were observed.
Financial Disclosure:
NONE
