Bacterial adhesion depends on the surface roughness of intraocular lens

Session Details

Session Title: Endophthalmitis/Ocular Infections

Session Date/Time: Wednesday 09/09/2015 | 08:00-09:30

Paper Time: 08:24

Venue: Room 8

First Author: : P.Biswas INDIA

Co Author(s): :    A. Paul   D. Unnikrishnan   P. Biswas   K. Chowdhury   S. Jana   N. Chandak     

Abstract Details

Purpose:

To assess the surface roughness properties of different intraocular lenses (IOLs) and its association with bacterial adhesion and to provide a better insight in the interactions between the IOL biomaterials and pathogenic bacteria.

Setting:

B B Eye Foundation, Kolkata and Indian Institute of Technology,Kharagpur, India

Methods:

2 sets of 5 IOL models of different biomaterials available were included,1 set to determine average surface roughness & another set to study bacterial adhesion. •Atomic force microscopy: Tapping mode AFM used to examine the topological features of IOL optics. •Bacterial Strains: Explanted IOLs from eyes of patients with severe endophthalmitis were inoculated. Bacterial strain was identified using 16s rRNA gene sequencing. •Growth kinetics and pH curve: pH and growth of bacterial culture monitored at 4 hour intervals. Bacterial growth kinetics investigated spectrophotometrically. •Adherence of Bacteria: Assessment on surface of IOL by scanning electron microscopy (Jeol JSM-5800 Scanning Microscope, OXFORD).

Results:

The blast results showed a 99% match with Streptococcus epidermidis. Surface roughness parameters: Surface quality of the optics of different IOLs was found to be significantly different. Bacterial adhesions were found to increase with increasing roughness. Bacterial growth was highest on acrylic hydrophilic lens with highest average roughness (13.64±0.75) followed by silicone (8.42 ± 2.72), acrylic (1.99±0.04), acrylic hydrophobic (1.71 ± 0.02) and least on PMMA (0.14±0.01).

Conclusions:

No study till date has investigated the role of Surface Roughness of Intraocular Lenses in Bacterial Adhesion. Our study showed that the bacterial growth increased with increasing surface roughness of IOLs. We conclude that the reduction of the surface roughness of the IOLs may represent a promising approach to reduce the bacterial adhesion and growth and thereby possibly Endophthalmitis. Thus strict and mandatory guidelines to manufacturers of intraocular lenses is of paramount importance to ensure proper standardization of intraocular lenses and thereby ensuring safety to our patients.

Financial Interest:

NONE