Novel contact lens disinfection solution with broad antimicrobial activity and low cytotoxicity

Session Details

Session Title: Special Cases I

Session Date/Time: Sunday 06/09/2015 | 14:30-16:00

Paper Time: 15:04

Venue: Room 10

First Author: : R.Metzinger USA

Co Author(s): :    R. Reimers   S. Killeen   C. Roy              

Abstract Details

Purpose:

Regular use of contact lens without proper hygienic maintenance can lead to multiple pathologies of the eye, including microbial keratitis. Current commercial contact lens disinfection solutions (CLDS) are developed for regular cleaning and antimicrobial disinfection to minimize the risks of potential adverse reactions associated with contact lens wear. A properly formulated CLDS ideally combines antimicrobial disinfection without inducing ocular cytotoxicity. A novel CLDS (SOL01) was specifically developed to be broadly effective against multiple microbial pathogens by inclusion of multiple chemical stressors rather than a single disinfection agent while simultaneously exerting minimal cytotoxicity upon tissues upon contact.

Setting:

Tulane University School of Medicine, New Orleans (Department of Microbiology and Immunolog and Department of Ophthalmology)

Methods:

Relative antimicrobial disinfection and cytotoxicity using ISO testing protocols 14729 (antimicrobial) and 10993 (cytotoxicity) was performed using the novel formulation (SOL01) in conjunction with three commercially-available CLDS as comparators. ISO-required battery of microorganisms (S. aureus, P. aeruginosa, S. marcesens, C. albicans, and F. solani) with contact times of 1, 2, and 4 hours were used to assess disinfection potential of each. Vero76 monkey kidney cells and XTT proliferation assay were used to assess in vitro cytotoxicity of each CLDS.

Results:

The disinfection potential of SOL01 in the ISO-specific antimicrobial assay demonstrated no bacterial/fungal plate growth for any of the microorganisms tested as early as 1 hour contact time. All comparator CLDS, in contrast, showed significant growth at 1 and 2 hour contact times, with only slight reductions of bacterial or fungal growth after 4 hours of contact time. Two of the three comparator CLDS exceeded 90% cell death at 1:1, requiring at least a 1:4−1:8 dilution in order to minimize cytotoxicity from contact. In contrast, SOL01 showed minimal cell death at 1:1, and required no dilutions to demonstrate low cytotoxicity.

Conclusions:

The newly-formulated CLDS (SOL01) combines broad spectrum antimicrobial killing against an array of microorganisms while maintaining minimal cytotoxicity on target tissues as demonstrated in vitro. The performance of the novel SOL01 in the two ISO-recommended standard assays exceeded the three commercially-available comparator CLDS. Future development of SOL01 and use for maintaining hygiene of contact lens wearers may further reduce unwanted toxic effects and ocular infections from inadvertent microbial contamination.

Financial Interest:

NONE