Posters

Determination of a specific marker of apoptosis in patients with retrobulbar neuritis on the background of multiple sclerosis

Poster Details

First Author: G.Khamraeva UZBEKISTAN

Co Author(s):    H. Kamilov   M. Kasimova                 

Abstract Details

Purpose:

To determine the specific apoptosis markers in patients with optic neuritis on the background of multiple sclerosis

Setting:

Tashkent Institute of Postgraduated Medical Education, Uzbekistan

Methods:

The study included 32 patients (52 eyes) with retrobulbar neuritis (RBN) against multiple sclerosis (MS). The control group consisted of 10 somatically healthy individuals. The level of neuron specific enolasa (NSE) in the tear fluid (TF) and blood serum (BS) was determined by enzyme-linked immunosorbent assay on a Cobas e 411 automated electrochemiluminescent immunoassay for chemiluminescent immunoassay (Roche Diagnostics, Switzerland). Statistical data processing was performed using the Statistica-10.0 program.

Results:

Patients with RBN often complained of a sharp decrease in visual acuity. According to perimetry, relative central scotomas were determined in 24 eyes (46.2%). The NSE content in the TF was increased compared with the control group and amounted to an average of 33.55 ± 2.45 ng /ml (p <0.01), although it remained within normal limits in serum (14.76 ± 0.76). That is, in patients there was a 2-fold increase in NSE level compared with the control group, which may be associated with the onset of destructive processes in the nervous tissue and the gradual death of neurons during neurodegeneration.

Conclusions:

Registration of the NSE level helps to indirectly make assumptions about the severity of the disease and predict the outcome, since the clinical picture and data from traditional research methods do not always reflect the degree of damage to the optic nerve. Thus, along with clinical signs, to assess the prognosis of disorders in RBN, it is necessary to determine NSE in the TF as a subclinical marker of the neurodegenerative process. Its sensitivity was 78%, specificity - 72%.

Financial Disclosure:

None