Posters

Ultrastructural and immunohistochemical changes in the primary and secondary lenticles after femtosecond technology Smile

Poster Details

First Author: O.Pisarevskaya RUSSIA

Co Author(s):    T. Iureva   E. Likhoshvay   A. Shchuko              

Abstract Details

Purpose:

To carry out ultrastructural and immunohistochemical studies of the lenticles obtained after Smile and repeated Smile surgery, which performing to correct residual myopia

Setting:

Irkutsk Branch of S. Fyodorov Eye Microsurgery Federal State Institution, Irkutsk, Russia; Department of Cell Infrastructure of Siberian Branch of the Russian Academy of Sciences

Methods:

We studied 6 lenticles obtained when correcting moderate myopia in Fast mode and 4 lenticles obtained when correcting residual myopia, which was performed no earlier than 12 months after the first operation. It is important to note that the secondary lenticular investigated for the first time. myopia in the original way, which is a prototype of the Smile operation. Scanning, transmission and laser confocal microscopy of the lenticular with additional visualization of actin microfilaments, lipids and cell nuclei with digital image processing.

Results:

Primary lenticular. On lenticular surfaces on end edge ruptures of collagen fibers and fibrils were revealed, in deeper parts of lenticule collagen fibers, diameter and position were not changed. In stroma, keratocytes that were in normal state were determined, on surface - cells with changes in form of vesicles appearance, increased granularity, cytoskeletal filaments. In most cases, chromatin is detected in nucleus. Appearance of myofibroblast marker F-actin. Secondary lenticular. In thickness from side of intrastromal space, only normal keratocytes were visualized. In keratocytes neutral fats located perinuclear or in one of cellular processes, extracellular matrix were detected

Conclusions:

Femtosecond stroma destruction during Smile surgery is accompanied by localized damage to collagen fibers and other elements of the extracellular matrix. Degenerative changes in keratocytes are reversible. The activation of keratocytes with their acquisition of myofibroblast function and the transition of cells to fat metabolism can be considered as a process of adaptation to surgical trauma

Financial Disclosure:

None