Posters

Endothelial cell loss following lamellar keratoplasty graft preparation: Femto-DSEK vs DSAEK

Poster Details

First Author: N.Shilova RUSSIA

Co Author(s):    B. Malyugin                    

Abstract Details

Purpose:

To evaluate and compare endothelial cells loss during and following graft preparation with Femtosecond laser (Femto-DSEK) versus microkeratome –(DSAEK).

Setting:

S. Fyodorov Eye Microsurgery State Institution, Moscow, Russia

Methods:

Retrospective study. Group A: 26 patients who underwent cataract surgery combined with Femto-DSEK. Group B: 28 patients who underwent cataract surgery combined with DSAEK. Intraoperative images before and after graft preparation were performed and using ImageJ software (National Institutes of Health, city,USA) the endothelial cell loss (ECL) was quantified. Outcomes measures were preoperative and postoperative endothelial cell density and BSCVA. Follow up period: 1 year.

Results:

The mean ECL after graft preparation significantly differed between group A to group B and was 17,2±2,7% and 11,5±4,1%, respectively (p<0,001). The mean ECL at 1 year following surgery significantly differ between group A to group B and was 65,7±7,7% and 58,5±8,1%, (p=0.02). A correlation was noted between ECL following graft preparation and ECL at 3 and 6 months after the surgery (r=0,718, p=0,012) (r=0,380; p=0,023). At one year follow-up there were significant difference in BSCVA between group A to group B: median acuity was 0.4 logmar (0.3;0.5) in group A and 0.3 (0.23;0.4) in group B (p=0.017).

Conclusions:

The use of femtosecond laser in comparison to microkeratome- DSAEK causes more endothelial cell loss during graft preparation and during the follow up time after the surgery. A correlation between ECL after graft preparation and ECL at 3 and 6 months after the surgery was noted.

Financial Disclosure:

None