Posters

Sleep deprivation and its impact on the quality of vision: a clinical and molecular analysis

Poster Details

First Author: N.Balakrishnan INDIA

Co Author(s):    T. S J   R. Shetty   S. Sethu   A. Ghosh           

Abstract Details

Purpose:

To understand the effect of lack of sleep on clinical metrics and molecular profile on the ocular surface.

Setting:

Narayana Nethralaya Super Speciality Hospital Bangalore India

Methods:

30 healthy volunteers (60 Eyes) underwent routine dry eye evaluation (Schirmer’s & Tear Break Up Time(TBUT) along with OSDI questionnaire, Lipid Layer assessment using LipiView Interferometer, Objective Scatter Index(OSI)using the OQAS (HD Analyzer) at Baseline, following Sleep Deprivation(for 24 hours)& after 3 nights of sufficient sleep(>8 hours). Eye wash &Tears (Schirmer’s strip) were collected & different cytokine levels were measured by multiplex ELISA (cytometric bead array) using a flow cytometer.

Results:

Sleep Deprivation resulted in significant decrease in Schirmer’s test value 1, TBUT & LipiView metrics and an increase in Scatter (OSI and Mean OSI) and OSDI. Tear cytokines and chemokines including IL-1a, IL-1b, IL-6, IL-12, IL-17F, MCP1, IP-10, FasL, ICAM1 etc., showed similar increase. The magnitude of tear cytokine changes was observed to be associated with the severity of TBUT, Schirmer’s test 1, Lipid Layer Thickness and Optical Scatter changes. A significant (p<0.05) reversal of these changes was observed following a period of adequate sleep.

Conclusions:

Sleep deprivation causes significant detrimental alteration in tear fluid dynamics/integrity, along with an increase in inflammatory cytokines on the ocular surface. It leads to increase in ocular scatter and decrease in lipid layer thickness, inducing dry eye & in turn leads to poor quality of vision. These improve, but do not return to normal levels even after 3 days of adequate sleep.

Financial Disclosure:

None