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Extracellular matrix modulation in the late spontaneous in-the-bag intraocular dislocated lenses

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First Author: J.Bisevac NORWAY

Co Author(s):    G. Petrovski                    

Abstract Details

Purpose:

Late spontaneous in-the-bag intraocular lens (IOL) dislocation presents a complication with increasing cumulative risk over post-cataract surgery years. We aimed to characterize the extracellular matrix (ECM) of lens capsules of 15 patients with spontaneous late in-the-bag IOL dislocation after the IOL exchange surgery.

Setting:

Center for Eye Research, Department of Ophthalmology, Oslo University Hospital and University of Oslo, Oslo, Norway

Methods:

Patient baseline characteristics were collected. Dislocated IOL-capsule complexes were analyzed for gene expression/ qRT-PCR and protein expression/ immunohistochemistry (IHC). Collagen gel contraction assay was done on primary cultured lens epithelial cells/myofibroblasts from healthy cadavers.

Results:

The most of patients had pseudoexfoliation syndrome (86.7%) with average age 82.5 ± 1.7 years (range 72-98), whereas time period between dislocation and cataract surgery was 11.2 ±1.6 years (range 3-25). IHC staining revealed extracellular matrix (ECM) positive for fibrous proteins: fibronectin, collagen I and V. In addition, ECM was positive for crystallin proteins. qRT-PCR findings were concomitant with the IHC. Contraction of collagen matrix was induced by cultured myofibroblasts.

Conclusions:

Fibrotic extracellular matrix of the capsular bag and crystallin accumulation through secondary cataract deposition may affect late in-the-bag spontaneous IOL dislocation

Financial Disclosure:

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