Free Papers

Evaluation of the Chemoprotective Effect of Resveratrol in Human Limbal Explant Culture

Free Paper Details

First Author: G.Dereli Can TURKEY

Co Author(s):    G. Taner   Ç. İnci Aydemir                 

Abstract Details

Purpose:

Mitomycin C (MMC) inhibits DNA synthesis during cell cycle and has long been used in ocular surgeries as a potential wound-healing modulator. Experimental studies have conclusively shown that MMC has a time- and dose-dependent non-specific cytotoxic activity on cells. The possible approach to protect cells from this damage is chemoprevention with antioxidants. Resveratrol (RSV) is a natural polyphenol phytoalexin compound and has long been considered to possess anti-oxidant and anti-inflammatory effects. In order to exploit the protective potential of RSV in corneal cells, we investigated the response of human limbal explant cultures to RSV and MMC alone and in combination.

Setting:

Experimental cell culture study.

Methods:

A total of 18 limbal explant tissues obtained from 3 corneal donors were placed on the 12 well tissue culture polystyrene plates and cultured for 14 days. Cell growth from limbal explants was observed by inverted phase contrast microscopy. The cytotoxic effects of RSV was studied by neutral red uptake assay. For the assesment of the genotoxic and antigenotoxic effects, basic alkaline technique of comet assay was performed.

Results:

It was found that the concentrations of RSV up to 100 M did not significantly affect the viability of outgrowth cells of limbal explant during 24 h exposure. When compared to negative control, all concentrations of RSV alone caused an increase in DNA strand breakage. Interestingly, 10 g/mL MMC alone caused similar tail intensity and tail moment values with RSV alone. On the other hand, RSV treatment in all doses seemed to decrease the DNA damage induced by either H2O2 and MMC.

Conclusions:

RSV is an attractive natural compound for the purpose of oxidative stress reduction in ocular surface and can be utilized as a supplement to promote ocular surface regeneration in vivo.

Financial Disclosure:

-