Analysis of collagen structural difference of human amniotic membrane and corneoscleral biomaterials using Raman spectroscopy

Poster Details

First Author: Jae-HoShin SOUTH KOREA

Co Author(s):                  

Abstract Details

Purpose:

To analyze the differences in the structure of collagen for three amniotic membranes (AM group) and corneoscleral tissue (EYE group) by Raman spectroscopy and histological analysis.

Setting:

: Department of Ophthalmology, Kyung Hee university Hospital at Gangdong, Kyung Hee University College of Medicine, Seoul, Korea

Methods:

Three AM biomaterials (n=4, each) including a human placental AM (pAM), a human reflected AM (rAM) and a preserved AM (gAM; Ambiodry2), and four human corneoscleral tissues were assessed by not only H&E (structure) and MT/PR (collagen) staining histlogically, also 785-nm laser Raman spectroscopy physicochemically.

Results:

AM groups showed a significant decreased collagen distribution compared to two EYE groups (p < 0.001). Particularly, pAM group showed significantly lower collagen density than rAM. However, we could obtain no histological information from gAM group. Raman spectra at amide-I (1655–1667 cm-1) and amide-III (1241–1276 cm-1) bands, which indicate the stabilization of a subfibrillar triple helical structure of collagen, showed a significant lower intensity in AM groups compared to EYE groups.

Conclusions:

All AM biomaterials showed lower collagen distribution than the corneoscleral tissue. Therefore it is likely that AM biomaterial with a weak collagen structure is good biomaterials for transplantation into the corneal tissue with a dense collagen structure. FINANCIAL INTEREST: NONE